A marine officer of the Cape Ray, a ship equipped to neutralize Syrian chemicals, shows a chemical protection suits to reporters, during a tour around the ship docked at the naval base of Rota used by the U.S, in Spain’s southwestern coast.

ISIS militants attacked Kurdish forces in Iraq with mustard gas last year, the first known use of chemical weapons in Iraq since the fall of Saddam Hussein, a diplomat said, based on tests by the global chemical weapons watchdog.

A source at the Organization for the Prohibition of Chemical Weapons (OPCW) confirmed that laboratory tests had come back positive for the sulphur mustard, after around 35 Kurdish troops were sickened on the battlefield last August.

The OPCW will not identify who used the chemical agent. But the diplomat, speaking on condition of anonymity because the findings have not yet been released, said the result confirmed that chemical weapons had been used by ISIS fighters.

The samples were taken after the soldiers became ill during fighting against ISIS militants southwest of Erbil, capital of Iraq’s autonomous Kurdish region.

The OPCW already concluded in October that mustard gas was used last year in neighboring Syria. ISIS has declared a “caliphate” in territory it controls in both Iraq and Syria and does not recognize the frontier.

Experts believe that the sulphur mustard either originated from an undeclared Syrian chemical stockpile, or that militants have gained the basic know how to develop and conduct a crude chemical attack with rockets or mortars.

Iraq’s chemical arsenal was mainly destroyed in the Saddam era, although U.S. troops encountered some old Saddam-era chemical munitions during the 2003-2011 U.S. occupation.

Syria gave up its own chemical weapons, including stockpiles of sulphur mustard, under international supervision after hundreds of civilians were killed with sarin nerve gas in a Damascus suburb in 2013, an attack Western countries blame on President Bashar al-Assad’s government, which denies it.

Sulphur mustard is a Class 1 chemical agent, which means it has very few uses outside chemical warfare. Used with lethal effectiveness in World War One, it causes severe delayed burns to the eyes, skin and respiratory tract.

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